anti clc 3 ab alone  (Alomone Labs)

Journal:

Article Title: Functional inhibition of native volume-sensitive outwardly rectifying anion channels in muscle cells and Xenopus oocytes by anti-ClC-3 antibody

doi: 10.1111/j.1469-7793.2001.0437i.x

Figure Lengend Snippet: A, phase contrast (left panel) and fluorescence micrographs (right panel) of NIH/3T3 cell transfected with gpClC-3-GFP. B, representative IgpClC-3 recorded from GFP-positive NIH/3T3 cells (as shown in A) under isotonic, hypotonic and hypertonic conditions over the range -100 to +120 mV. C, effects of anti-ClC-3 Ab on IgpClC-3 at ±80 mV when pipette solutions contained preabsorbed anti-ClC-3 Ab (control, ○) or anti-ClC-3 Ab alone (▪). D, mean current densities recorded from untransfected NIH/3T3 cells (blue, n = 38), gpClC-3-GFP-transfected cells dialysed with standard intracellular pipette solutions (green, n = 9), preabsorbed anti-ClC-3 Ab (black, n = 4), and anti-ClC-3 Ab alone (red, n = 9).

Article Snippet: Furthermore, in cardiac tissues endogenous ClC-3 protein expression has been confirmed using the same anti-ClC-3 Ab (Alomone Labs) in immunoblot and immunofluorescence experiments ( Britton et al. 2000 ). and illustrate the effects of intracellular dialysis of anti-ClC-3 Ab alone or antigen-preabsorbed anti-ClC-3 Ab on native VSOACs in guinea-pig cardiac and canine pulmonary arterial smooth muscle myocytes, respectively. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 2 caption a7 caption a8 Anti-ClC-3 Ab dialysis abolishes native VSOAC currents in guinea-pig cardiac myocytes A , native VSOAC currents at ±80 mV in myocytes exposed to isotonic and hypotonic solutions.

Techniques: Fluorescence, Transfection, Transferring

Journal:

Article Title: Functional inhibition of native volume-sensitive outwardly rectifying anion channels in muscle cells and Xenopus oocytes by anti-ClC-3 antibody

doi: 10.1111/j.1469-7793.2001.0437i.x

Figure Lengend Snippet: A, time course of VSOAC currents from two PASMCs dialysed with either anti-ClC-3 Ab (5 μg ml−1) preabsorbed with antigen (50 μg ml−1; ○), or anti-ClC-3 Ab alone (5 μg ml−1; •). Inset: Western blot analysis of native ClC-3 expression in isolated canine PASMCs. B, mean current densities in cells dialysed with either standard intracellular solutions (n = 11), preabsorbed anti-ClC-3 Ab (n = 4) or anti-ClC-3 Ab alone (n = 5).

Article Snippet: Furthermore, in cardiac tissues endogenous ClC-3 protein expression has been confirmed using the same anti-ClC-3 Ab (Alomone Labs) in immunoblot and immunofluorescence experiments ( Britton et al. 2000 ). and illustrate the effects of intracellular dialysis of anti-ClC-3 Ab alone or antigen-preabsorbed anti-ClC-3 Ab on native VSOACs in guinea-pig cardiac and canine pulmonary arterial smooth muscle myocytes, respectively. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 2 caption a7 caption a8 Anti-ClC-3 Ab dialysis abolishes native VSOAC currents in guinea-pig cardiac myocytes A , native VSOAC currents at ±80 mV in myocytes exposed to isotonic and hypotonic solutions.

Techniques: Western Blot, Expressing, Isolation

Journal:

Article Title: Functional inhibition of native volume-sensitive outwardly rectifying anion channels in muscle cells and Xenopus oocytes by anti-ClC-3 antibody

doi: 10.1111/j.1469-7793.2001.0437i.x

Figure Lengend Snippet: A, native VSOAC currents at ±80 mV in myocytes exposed to isotonic and hypotonic solutions. Aa, pipette solutions contained preabsorbed anti-ClC-3 Ab (control, ○) or anti-ClC-3 Ab alone (▪). Ab, mean current densities for preabsorbed anti-ClC-3 Ab (□, n = 3) or anti-ClC-3 Ab alone (▪, n = 5) at times indicated. B, same as A, except cells were preswelled by pre-exposure to hypotonic bath solutions prior to membrane rupture and dialysis; preabsorbed anti-ClC-3 Ab (□, n = 4), and anti-ClC-3 Ab alone (▪, n = 5).

Article Snippet: Furthermore, in cardiac tissues endogenous ClC-3 protein expression has been confirmed using the same anti-ClC-3 Ab (Alomone Labs) in immunoblot and immunofluorescence experiments ( Britton et al. 2000 ). and illustrate the effects of intracellular dialysis of anti-ClC-3 Ab alone or antigen-preabsorbed anti-ClC-3 Ab on native VSOACs in guinea-pig cardiac and canine pulmonary arterial smooth muscle myocytes, respectively. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 2 caption a7 caption a8 Anti-ClC-3 Ab dialysis abolishes native VSOAC currents in guinea-pig cardiac myocytes A , native VSOAC currents at ±80 mV in myocytes exposed to isotonic and hypotonic solutions.

Techniques: Transferring

Journal:

Article Title: Functional inhibition of native volume-sensitive outwardly rectifying anion channels in muscle cells and Xenopus oocytes by anti-ClC-3 antibody

doi: 10.1111/j.1469-7793.2001.0437i.x

Figure Lengend Snippet: A, Western blot analysis of native ClC-3 expression in oocytes. B, 4-phorbol 12,13-dibutyrate (PDBu) inhibition of native VSOAC currents in oocytes (-100 to +120 mV). C, time course of VSOAC activation in response to hypotonic bath solutions from a non-injected control oocyte (○) and an oocyte injected with anti-ClC-3 Ab (15 μg ml-1; •) at time shown. Cells were held at -30 mV for 30 ms, hyperpolarized to -100 mV for 210 ms and then depolarized to +100 mV for 210 ms, repetitively at 2 Hz. D, mean current densities from control oocytes (n = 10), oocytes injected with anti-ClC-3 Ab alone (15 μg ml-1) (n = 4) or injected with preabsorbed anti-ClC-3 Ab (150 μg ml-1) (n = 4). Peak current densities were measured after 5 min exposure to isotonic solutions, and after 85 min exposure to hypotonic solutions.

Article Snippet: Furthermore, in cardiac tissues endogenous ClC-3 protein expression has been confirmed using the same anti-ClC-3 Ab (Alomone Labs) in immunoblot and immunofluorescence experiments ( Britton et al. 2000 ). and illustrate the effects of intracellular dialysis of anti-ClC-3 Ab alone or antigen-preabsorbed anti-ClC-3 Ab on native VSOACs in guinea-pig cardiac and canine pulmonary arterial smooth muscle myocytes, respectively. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 2 caption a7 caption a8 Anti-ClC-3 Ab dialysis abolishes native VSOAC currents in guinea-pig cardiac myocytes A , native VSOAC currents at ±80 mV in myocytes exposed to isotonic and hypotonic solutions.

Techniques: Western Blot, Expressing, Inhibition, Activation Assay, Injection